22 accepted papers
Various forms of biohybrid robots have been developed; however, creating robots with multiple degrees of freedom remains a challenging task. In this paper, we developed a multi-joint biohybrid robot by using skeletal muscle tissue. To achieve this, we first developed a modular bio-actuator actuated
There has been an increasing demand for microscopic work using optical microscopes and micromanipulators for applications in various fields. However, microinjection requires skilled operators, and the considerable shortage of experts has become a recent challenge. We overcome this challenge by propo
In this paper, we fabricated a soft sensor based on PEDOT:PSS for thin film structure. The developed soft sensor can measure the contraction force at real time to be embedded in a modular bio-actuator [1]. The modular actuator generated contraction forces at 0.3 mN when applying electric pulse stimu…
In spite of recent high attention of the biohybrid robot system, the previous researches focused on actuation system depend on simple on/off control without feedback control. To solve this problem, we proposed a soft sensor for feedback control of a bio-actuator driven by skeletal muscle. The propos…
The microinjection technique, in which DNA is injected into cells, is widely applied in fields such as biology, medicine, and pharmacy. However, the implementation of the microinjection process requires skill and, thus, it is necessary to improve the operability. To this end, this study proposes a m
Microinjection technology is applied widely in biomedical research for the purposes of gene manipulation and microinsemination. Generally, microinjection is performed under an optical microscope environment through image presentation of the targets. To perform the microinjection process, it is neces…
In this paper, we have constructed actively perfusable multiple hepatic lobule-like vascular networks in a 3D cellular structure by using magnetic tweezers. Without well-organized channel networks, cells in a large 3D tissue cannot receive nutrients and oxygen from the channel, and therefore, the ce…
Image compression is a fundamental research field and many well-known compression standards have been developed for many decades. Recently, learned compression methods exhibit a fast development trend with promising results. However, there is still a performance gap between learned compression algor…
Lossless image compression is an important task in the field of multimedia communication. Traditional image codecs typically support lossless mode, such as WebP, JPEG2000, FLIF. Recently, deep learning based approaches have started to show the potential at this point. HyperPrior is an effective tech…
In this paper, we have fabricated a multilayered hepatic lobule-like vascular network in a 3D tissue using a heptapole magnetic tweezer. The tissue consists of cell-laden hydrogels with 3D channel networks. To fabricate multilayered channel system, magnetic hydrogel fibers were manipulated by a magn…
Compression has been an important research topic for many decades, to produce a significant impact on data transmission and storage. Recent advances have shown a great potential of learning based image and video compression. Inspired from related works, in this paper, we present an image compression…
Fabrication of vascular network is an important research for transporting required nutrients and oxygen to the artificial tissues. In this paper, we propose a novel method to construct a hepatic lobule-like vascular network in a 3D cellular structure. The network is simply constructed by three types…
This study presents the high-precision microinjection of fluorescent micro-gel beads into Caenorhabditis elegans trapped in a suction microchannel. In our previous works, we demonstrated survival microinjection by a conventional micromanipulation technique. However, the focal planes differed between…
In this letter, we conducted a new method to fabricate channels inside cell structures for tissue engineering applications. A cell-embedded hydrogel is locally melted to fabricate channels inside cell structures. The fabricated channels can be used as vascular-like networks to supply nutrition and o
In this research, we fabricated movable toroidal cell structures inside a microfluidic device for tissue engineering applications. A photo-crosslinkable biodegradable hydrogel gelatin methacrylate (GelMA) was employed to encapsulate biological cells for assembling cell structures. The UV light power…
Constructing 3D cell module with certain shape and high cell density is a very important issue for artificial tissue engineering. In this letter, we present a novel method of fabricating compact microtissue with gear-like shape by electrodepositing of Ca <sup xmlns:mml="http://www.w3.org/1998/Math/M
Alginate hydrogel microfibers are a promising cell-laden module for three-dimensional (3-D) assembly to build cellular structures. However, it is still a challenge to manipulate them for microassembly. In this letter, we report a novel magnetic control method to handle this challenge. To enhance the
This paper presents a novel In situ nanomanipulation integrated with scanning electron microscope- computed tomography (SEM-CT) imaging system for 3D nanomanipulation. In our previous works, a nanorobotic manipulation system was established inside an environmental-SEM (E-SEM) for water-contained sam
In this paper, we propose a new method to assemble microstructures with biological cells towards in vitro 3D cellular structures. The proposed assembly method uses self-assembly process of magnetized toroidal microstructures. Biocompatible toroidal hydrogel microstructures are prepared by electrodep…
In this study, cell-laden alginate-poly-L-lysine (PLL) hydrogel structures with arbitrary shapes were constructed based on electrodeposition method. Electrolysis of water in alginate solutions with calcium carbonate particles induced alginate gelation on micro-patterned anode electrode, and cell-lad…
In this paper, we conducted a new method to assemble microstructures inside a droplet named “Lab in a Droplet (LiD)”. The method can realize the assembly of micro-nano structures inside a droplet size. The surface tension is used to assemble microstructures automatically. Micro-scale or nano-scale o…
This study presents the survival microinjection into Caenorhabditis elegans (C. elegans) with in vivo observation based on micromanipulation. The microinjections were achieved with micro-gel beads which are enable to encapsulate chemicals for injection. In this study, a fluorescent material was used…