7 accepted papers
Spatially resolved single-cell analysis of plant root tissues requires isolating target cells while preserving their original three-dimensional tissue context, which remains challenging due to microscopic size, structural fragility, and dense spatial distribution. This article presents an automated
We developed a microscopic cell/tissue extraction device that employed a translational/rotational piezoelectric impact drive mechanism (Piezo IDM). To perform the correlation between gene expression and localized tissue sample at the micrometer scale, the system inserted a knife-edged glass capillar…
This study proposes a novel strategy for cross-scale position of robotic micromanipulation. The strategy utilizes multiple-scale augmented reality (AR) markers for locating the robotic manipulator on different scales. The macro-marker (3.0 cm-per side, 5 mm×5 mm each square) is applied to position t
This study proposes a novel movable microfluidic chip in which a microfluidic chip is integrated into a robotic manipulator for manipulating oocytes. The microfluidic device has the ability to release a single oocyte with a gap effect. The robotic manipulator can control the position of the microflu…
This paper presented the three-dimensional oocyte manipulation system for the two-electrode voltage clamp (TEVC) experiment under stereomicroscopy. We firstly developed a sequential calibration method to correlate the workspace of the stereomicroscopy with the image and the micromanipulator. Even th…
This paper presents a system that picks up protoplasts produced by removing the surrounding cell wall of root cells while preserving their positional information. The fundamental concept of this system involves scanning the root tip over time using a confocal microscopy to measure the positional inf…
A microactuator that exhibited the variable stiffness function and the strain control was demonstrated by using the shape memory alloy (SMA), shape memory polymer (SMP), and the strain-sensitive stretchable heater polymer of multi-walled carbon nanotube/ionic liquid (IL): polydimethylsiloxane. The S