10 accepted papers
Spatially resolved single-cell analysis of plant root tissues requires isolating target cells while preserving their original three-dimensional tissue context, which remains challenging due to microscopic size, structural fragility, and dense spatial distribution. This article presents an automated
We developed a microscopic cell/tissue extraction device that employed a translational/rotational piezoelectric impact drive mechanism (Piezo IDM). To perform the correlation between gene expression and localized tissue sample at the micrometer scale, the system inserted a knife-edged glass capillar…
This study proposes a novel strategy for cross-scale position of robotic micromanipulation. The strategy utilizes multiple-scale augmented reality (AR) markers for locating the robotic manipulator on different scales. The macro-marker (3.0 cm-per side, 5 mm×5 mm each square) is applied to position t
This study proposes a novel movable microfluidic chip in which a microfluidic chip is integrated into a robotic manipulator for manipulating oocytes. The microfluidic device has the ability to release a single oocyte with a gap effect. The robotic manipulator can control the position of the microflu…
This paper presented the three-dimensional oocyte manipulation system for the two-electrode voltage clamp (TEVC) experiment under stereomicroscopy. We firstly developed a sequential calibration method to correlate the workspace of the stereomicroscopy with the image and the micromanipulator. Even th…
A microactuator that exhibited the variable stiffness function and the strain control was demonstrated by using the shape memory alloy (SMA), shape memory polymer (SMP), and the strain-sensitive stretchable heater polymer of multi-walled carbon nanotube/ionic liquid (IL): polydimethylsiloxane. The S
The automation of the experimental process and evaluation method is required to screen chemical substances in the fields of biology and chemistry. In the case with biological evaluations, the automation of the mechanical probing has been considered, but the measurement method should possess versatil
This paper proposed a technique to introduce the membrane protein into the lab-on-chip analysis system having a planar lipid bilayer. The proposed technique utilized a dielectrophoretic(DEP) force generated by the asymmetric configuration of the solid electrodes on the aqueous buffer separator. By a…
Robot-integrated microfluidic chip is a promising tool to realize mechanical characterization of a single cell with high throughput and high accuracy. The microfluidic chip used in our system has a pair of mechanical microprobes to measure deformation and reaction force of cells. In our previous stu
This paper proposes the method for mechanical characterization of floating cells on a microfluidic chip. We measured the reactive force of cells on the chip by applying mechanical deformation to the cells. Particularly we focused on the method to improve the sensing resolution of the probe position…